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Table 2 GluDH activity of cell-free extracts prepared from wild-type strains and their recombinants

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Microorganism GluDH activitya
U/g-CDW U/mg-total protein
B. subtilis 168 0.99 ± 0.44 0.008 ± 0.001
B. subtilis 3C5N 0.22 ± 0.05 0.002 ± 0.000
B. amyloliquefaciens SB5 15.8 ± 1.90 0.166 ± 0.085
B. subtilis B-7b 0.02 ± 0.02 0.0002 ± 0.0002
B. subtilis BSc 6.84 ± 0.59 0.020 ± 0.003
B. subtilis BAd 1601 ± 146 6.24 ± 0.39
  1. aThe activity was determined using Tris–Cl (pH 8) at 37 °C
  2. b B. subtilis B-7 was a recombinant B. subtilis 3C5N overexpressing P450 BM3 F87V (Siriphongphaew et al. 2012)
  3. c B. subtilis BS was a recombinant B. subtilis 168 overexpressing GluDH-BS from itself
  4. d B. subtilis BA was a recombinant B. subtilis 168 overexpressing GluDH-BA from B. amyloliquefaciens SB5