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Table 2 GluDH activity of cell-free extracts prepared from wild-type strains and their recombinants

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Microorganism

GluDH activitya

U/g-CDW

U/mg-total protein

B. subtilis 168

0.99 ± 0.44

0.008 ± 0.001

B. subtilis 3C5N

0.22 ± 0.05

0.002 ± 0.000

B. amyloliquefaciens SB5

15.8 ± 1.90

0.166 ± 0.085

B. subtilis B-7b

0.02 ± 0.02

0.0002 ± 0.0002

B. subtilis BSc

6.84 ± 0.59

0.020 ± 0.003

B. subtilis BAd

1601 ± 146

6.24 ± 0.39

  1. aThe activity was determined using Tris–Cl (pH 8) at 37 °C
  2. b B. subtilis B-7 was a recombinant B. subtilis 3C5N overexpressing P450 BM3 F87V (Siriphongphaew et al. 2012)
  3. c B. subtilis BS was a recombinant B. subtilis 168 overexpressing GluDH-BS from itself
  4. d B. subtilis BA was a recombinant B. subtilis 168 overexpressing GluDH-BA from B. amyloliquefaciens SB5