Table 1 Primers used in this study
No. | Primer | Sequence |
|---|---|---|
1 | gdh-bs-F-BamHI | 5′CCGGATCCATGTATCCGGATTTAAAAGGAA3′ |
2 | gdh-ba-F-BamHI | 5′CCGGATCCATGTACACGGATTTAAAAGGAA3′ |
3 | gdh-R-KpnI | 5′CTGGTACCTTATCCGCGGCCTG3′ |
4 | gdh-ba-F-NdeI-His | 5′CGCCATATGCACCACCACCACCACCACTACACGGATTTAAAAGGAAAAG3′ |
5 | gdh-R-XhoI | 5′CCGCTCGAGTTATCCGCGGCCTGCCTGGAATG3′ |
6 | gdh-bs-F-BsrGI | 5′CCTGTACAATGTATCCGGATTTAAAAGGAA3′ |
7 | gdh-ba-F-BsrGI | 5′CCTGTACAATGTACACGGATTTAAAAGGAA3′ |