Fig. 1

Ribo-SPIA method amplified both NoV GI and GII genomes. a NoV GI-specific real-time RT-PCR on Norwalk virus before (undiluted) and after Ribo-SPIA (10-fold dilution); ∆Ct = 9 which equals a 5120-fold increase in signal. b NoV GII-specific real-time RT PCR on NoV 186 before (undiluted) and after Ribo-SPIA (10-fold dilution). ∆Ct = 12 which equals 40960-fold increase in signal. Negative control for both Ribo-SPIA and PCR was nuclease free water