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Table 1 Comparison of different media on total protein and biomass production by E. coli BL21 (DE3)/pAV01

From: High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization

Media

Total proteins (g/L)

Biomass (g/L)

Total protein/biomass ratio

LB

2.10 ± 0.12

10.22 ± 0.27

0.21

Terrific broth

3.02 ± 0.25

14.60 ± 0.95

0.21

M9 minimal medium

1.40 ± 0.11

7.45 ± 0.50

0.19

Modified minimal medium

1.63 ± 0.11

8.25 ± 0.38

0.20

Riesenberg mineral medium

1.57 ± 0.07

7.58 ± 0.33

0.21