Fig. 4From: High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterization a Ouchterlony radial immunodiffusion showing cross-reactivity of IgG with commercial SpA and purified AviPure. Upper Center well (X) contain BSA. Upper row wells (R1–R5) contain different concentration of commercial SpA (concentration 5, 1, 0.5 and 0.1 mg/mL), while middle (A1:1–4) and lower rows (A2:1–4) contain concentration (5, 1, 0.5 and 0.1 mg/mL) of purified AviPure. b Western blot analysis of of protein As. Western blot was carried out with Goat anti-protein A polyclonal antibodies HPR conjugate. Lanes 1 and 2; Commercial SpA, Lanes 3 and 4 AviPure eluted from SECBack to article page