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Table 1 Different media for the production of Surfactin with Bacillus subtilis

From: Enhancement of Surfactin yield by improving the medium composition and fermentation process

  Cooper medium Modified after Cooper Further optimized
C 40 g/L glucose 40 g/L glucose 8 g/L glucose
N 50 mM NH4NO3 100 mM NH4Cl 50 mM (NH4)2SO4
Mg 0.8 mM MgSO4 0.8 mM MgSO4 0.8 mM MgSO4
Buffer 30 mM KH2PO4 30 mM KH2PO4 30 mM KH2PO4
40 mM Na2HPO4 40 mM Na2HPO4 40 mM Na2HPO4
Trace elements 0.004 mM Na2EDTA 0.004 mM Na2EDTA 0.008 mM Na3citrate
0.007 mM CaCl2 0.007 mM CaCl2 0.007 mM CaCl2
0.004 mM FeSO4 0.004 mM FeSO4 0.004 mM FeSO4
0.001 mM MnSO4 0.001 mM MnSO4 0.001 mM MnSO4
  1. The original medium after Cooper et al. is shown in the first column (A). A slightly changed version of this medium was used throughout most experiments for previous studies (B). Hereby, the nitrogen source NH4NO3 was replaced by NH4Cl. During the current study the medium was further optimized to yield more Surfactin (C), employing less glucose (8 g/L), (NH4)2SO4 and Na3citrate