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Table 1 Different media for the production of Surfactin with Bacillus subtilis

From: Enhancement of Surfactin yield by improving the medium composition and fermentation process

 

Cooper medium

Modified after Cooper

Further optimized

A

B

C

C

40 g/L glucose

40 g/L glucose

8 g/L glucose

N

50 mM NH4NO3

100 mM NH4Cl

50 mM (NH4)2SO4

Mg

0.8 mM MgSO4

0.8 mM MgSO4

0.8 mM MgSO4

Buffer

30 mM KH2PO4

30 mM KH2PO4

30 mM KH2PO4

40 mM Na2HPO4

40 mM Na2HPO4

40 mM Na2HPO4

Trace elements

0.004 mM Na2EDTA

0.004 mM Na2EDTA

0.008 mM Na3citrate

0.007 mM CaCl2

0.007 mM CaCl2

0.007 mM CaCl2

0.004 mM FeSO4

0.004 mM FeSO4

0.004 mM FeSO4

0.001 mM MnSO4

0.001 mM MnSO4

0.001 mM MnSO4

  1. The original medium after Cooper et al. is shown in the first column (A). A slightly changed version of this medium was used throughout most experiments for previous studies (B). Hereby, the nitrogen source NH4NO3 was replaced by NH4Cl. During the current study the medium was further optimized to yield more Surfactin (C), employing less glucose (8 g/L), (NH4)2SO4 and Na3citrate