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Table 2 Physicochemical characteristics of the inoculum, cow feces and inoculum-substrate mixture at beginning of digestion cycle

From: A start-up of psychrophilic anaerobic sequence batch reactor digesting a 35 % total solids feed of dairy manure and wheat straw

Cycle Type Acetate (g kg−1) Propionate (g kg−1) Butyrate (g kg−1) pH Alkalinity (g kg−1 as CaCO3) TCOD (g kg−1) TS (%) VS (%)
1 Inoculum 0.77 ± 0.47 0.24 ± 0.21 0.28 ± 0.08 7.3 ± 0.0 11.5 ± 0.55   11.0 ± 0.6 9.3 ± 0.5
Feces 4.5 1.1 0.93 5.89   148.1 13.29 11.9
ISM 1.1 ± 0.02 0.25 ± 0.02 0.29 ± 0.02 7.1 ± 0.0    14.1 ± 0.4 12.0 ± 0.6
2 Inoculum 0.84 ± 0.50 0.32 ± 0.22 0.26 ± 0.07 7.4 ± 0.1 11.8 ± 0.65   12.3 ± 0.4 10.6 ± 0.4
Feces 3.4 0.88 1.39 6.18   148.1 13.29 11.9
ISM 1.2 ± 0.05 0.28 ± 0.02 0.10 ± .01 7.0 ± 0.1    15.3 ± 0.1 13.4 ± 0.1
3 Inoculum 0.25 ± 0.03 0.0 ± 0.0 0.0 ± 0.0 7.3 ± 0.1 10.0 ± 1.8   13.8 ± 0.7 11.8 ± 0.6
Feces 3.82 1.15 0.80 6.3   195.2 13.24 11.9
ISM 1.79 ± 166.4 0.25 ± 135.5 0.08 ± 0.01 7.0 ± 0.2 9.6 ± 0.56   16.4 ± 0.3 14.3 ± 0.3
4 Inoculum 0.244 ± 0.01 0.01 ± 0.01 0.14 ± 0.02 7.6 ± 0.0    14.5 ± 0.3 12.4 ± 0.3
Feces 3.99 1.05 0.65 6.64   173.5 13.22 11.8
ISM 1.16 ± 0.08 0.24 ± 0.03 0.12 ± 0.11 7.2 ± 0.1    17.0 ± 0.6 14.8 ± 0.5
5 Inoculum NA NA NA 7.3 ± 0.3   NA 15.2 ± 0.3 13.4 ± 0.5
Feces 4.31 1.10 0.78 5.1   173.5 12.8 11.3
ISM 0.86 ± 0.07 0.23 ± 0.03 0.19 ± 0.04 7.0 ± 0.1    18.7 ± 0.7 16.5 ± 0.7
6 Inoculum 0.13 ± 0.01 10.02 ± 0.0 0.0 ± 0.0 7.5 ± 0.5    16.8 ± 0.1 14.6 ± 0.1
Feces 3.48 1.47 2.37 6.5   183.2 12.8 11.3
ISM 0.56 ± 0.13 0.11 ± 0.05 0.11 ± 0.03 7.0 ± 0.2    19.3 ± 0.5 17.0 ± 0.5
7 Inoculum 0.18 ± 0.04 0.04 ± 0.0 0.06 ± 0.01 7.5 ± 0.1    17.6 ± 0.3 15.3 ± 0.2
Feces 3.69 1.00 0.72    183.2 12.8 11.3
ISM 0.35 ± 0.13 0.05 ± 0.01 0.06 ± 0.01 7.0 ± 0.2 9.4 ± 0.42   20.7 ± 0.4 18.2 ± 0.4
8 Inoculum 0.18 ± 0.03 0.03 ± 0.00 0.21 ± 0.03 7.6 ± 0.1    18.7 ± 0.3 16.2 ± 0.3
Feces 1.54 0.46 0.26 6.95 5.5 184.2 12.53 11.2
ISM 0.93 ± 0.06 0.28 ± 0.03 0.29 ± 0.03 7.1 ± 0.1    20.7 ± 1.3 18.0 ± 0.9
9 Inoculum 0.13 ± 0.02 0.03 ± 0.01 0.18 ± 0.02 7.6 ± 0.1    19.5 ± 0.1 17.0 ± 0.1
Feces 1.54 0.46 0.26 6.95 5.5 184.2 12.53 11.2
ISM 0.59 ± 0.08 0.11 ± 0.04 0.23 ± 0.04 7.1 ± 0.1    21.5 ± 0.4 19.0 ± 0.4
10 Inoculum 0.18 ± 0.04 0.05 ± 0.02 0.11 ± 0.03 7.7 ± 0.1    20.0 ± 0.2 17.4 ± 0.2
Feces 5.0 1.34 2.22 6.17   220.5 16.18 14.3
ISM 0.53 ± 0.11 0.05 ± 0.01 0.17 ± 0.03 7.2 ± 0.1    21.4 ± 0.4 18.7 ± 0.3
11 Inoculum 1.15 ± 0.03 0.03 ± 0.02 9.9 ± 17.1 7.7 ± 0.1 9.7 ± 0.32   20.4 ± 0.4 17.6 ± 0.4
Feces 5.0 1.34 2.22 6.17   220.5 16.18 14.3
ISM 0.23 ± 0.04 0.03 ± 0.02 0.0 ± 0.0 7.1 ± 0.3 9.6 ± 0.32   22.0 ± 0.7 19.3 ± 0.7
1-11 Wheat straw 1097 89.0 ± 0.0 85 ± 0.1
  1. ISM inoculum-substrate mixture