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Table 2 Physicochemical characteristics of the inoculum, cow feces and inoculum-substrate mixture at beginning of digestion cycle

From: A start-up of psychrophilic anaerobic sequence batch reactor digesting a 35 % total solids feed of dairy manure and wheat straw

Cycle

Type

Acetate (g kg−1)

Propionate (g kg−1)

Butyrate (g kg−1)

pH

Alkalinity (g kg−1 as CaCO3)

TCOD (g kg−1)

TS (%)

VS (%)

1

Inoculum

0.77 ± 0.47

0.24 ± 0.21

0.28 ± 0.08

7.3 ± 0.0

11.5 ± 0.55

 

11.0 ± 0.6

9.3 ± 0.5

Feces

4.5

1.1

0.93

5.89

 

148.1

13.29

11.9

ISM

1.1 ± 0.02

0.25 ± 0.02

0.29 ± 0.02

7.1 ± 0.0

  

14.1 ± 0.4

12.0 ± 0.6

2

Inoculum

0.84 ± 0.50

0.32 ± 0.22

0.26 ± 0.07

7.4 ± 0.1

11.8 ± 0.65

 

12.3 ± 0.4

10.6 ± 0.4

Feces

3.4

0.88

1.39

6.18

 

148.1

13.29

11.9

ISM

1.2 ± 0.05

0.28 ± 0.02

0.10 ± .01

7.0 ± 0.1

  

15.3 ± 0.1

13.4 ± 0.1

3

Inoculum

0.25 ± 0.03

0.0 ± 0.0

0.0 ± 0.0

7.3 ± 0.1

10.0 ± 1.8

 

13.8 ± 0.7

11.8 ± 0.6

Feces

3.82

1.15

0.80

6.3

 

195.2

13.24

11.9

ISM

1.79 ± 166.4

0.25 ± 135.5

0.08 ± 0.01

7.0 ± 0.2

9.6 ± 0.56

 

16.4 ± 0.3

14.3 ± 0.3

4

Inoculum

0.244 ± 0.01

0.01 ± 0.01

0.14 ± 0.02

7.6 ± 0.0

  

14.5 ± 0.3

12.4 ± 0.3

Feces

3.99

1.05

0.65

6.64

 

173.5

13.22

11.8

ISM

1.16 ± 0.08

0.24 ± 0.03

0.12 ± 0.11

7.2 ± 0.1

  

17.0 ± 0.6

14.8 ± 0.5

5

Inoculum

NA

NA

NA

7.3 ± 0.3

 

NA

15.2 ± 0.3

13.4 ± 0.5

Feces

4.31

1.10

0.78

5.1

 

173.5

12.8

11.3

ISM

0.86 ± 0.07

0.23 ± 0.03

0.19 ± 0.04

7.0 ± 0.1

  

18.7 ± 0.7

16.5 ± 0.7

6

Inoculum

0.13 ± 0.01

10.02 ± 0.0

0.0 ± 0.0

7.5 ± 0.5

  

16.8 ± 0.1

14.6 ± 0.1

Feces

3.48

1.47

2.37

6.5

 

183.2

12.8

11.3

ISM

0.56 ± 0.13

0.11 ± 0.05

0.11 ± 0.03

7.0 ± 0.2

  

19.3 ± 0.5

17.0 ± 0.5

7

Inoculum

0.18 ± 0.04

0.04 ± 0.0

0.06 ± 0.01

7.5 ± 0.1

  

17.6 ± 0.3

15.3 ± 0.2

Feces

3.69

1.00

0.72

  

183.2

12.8

11.3

ISM

0.35 ± 0.13

0.05 ± 0.01

0.06 ± 0.01

7.0 ± 0.2

9.4 ± 0.42

 

20.7 ± 0.4

18.2 ± 0.4

8

Inoculum

0.18 ± 0.03

0.03 ± 0.00

0.21 ± 0.03

7.6 ± 0.1

  

18.7 ± 0.3

16.2 ± 0.3

Feces

1.54

0.46

0.26

6.95

5.5

184.2

12.53

11.2

ISM

0.93 ± 0.06

0.28 ± 0.03

0.29 ± 0.03

7.1 ± 0.1

  

20.7 ± 1.3

18.0 ± 0.9

9

Inoculum

0.13 ± 0.02

0.03 ± 0.01

0.18 ± 0.02

7.6 ± 0.1

  

19.5 ± 0.1

17.0 ± 0.1

Feces

1.54

0.46

0.26

6.95

5.5

184.2

12.53

11.2

ISM

0.59 ± 0.08

0.11 ± 0.04

0.23 ± 0.04

7.1 ± 0.1

  

21.5 ± 0.4

19.0 ± 0.4

10

Inoculum

0.18 ± 0.04

0.05 ± 0.02

0.11 ± 0.03

7.7 ± 0.1

  

20.0 ± 0.2

17.4 ± 0.2

Feces

5.0

1.34

2.22

6.17

 

220.5

16.18

14.3

ISM

0.53 ± 0.11

0.05 ± 0.01

0.17 ± 0.03

7.2 ± 0.1

  

21.4 ± 0.4

18.7 ± 0.3

11

Inoculum

1.15 ± 0.03

0.03 ± 0.02

9.9 ± 17.1

7.7 ± 0.1

9.7 ± 0.32

 

20.4 ± 0.4

17.6 ± 0.4

Feces

5.0

1.34

2.22

6.17

 

220.5

16.18

14.3

ISM

0.23 ± 0.04

0.03 ± 0.02

0.0 ± 0.0

7.1 ± 0.3

9.6 ± 0.32

 

22.0 ± 0.7

19.3 ± 0.7

1-11

Wheat straw

1097

89.0 ± 0.0

85 ± 0.1

  1. ISM inoculum-substrate mixture