Table 1 Strains used in the study
From: Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica
Strains | Characteristic | References |
|---|---|---|
S. enterica sv. Typhimurium ATCC 14028 | Wild type | ATCC |
S. enterica sv. Typhimurium MHM21 | spvA::cat, I-SecI recognition site | This study |
S. enterica sv. Typhimurium MHM113 | Lacks virulence plasmid p14028 | This study |
Plasmids | ||
pWRG100 | cat, I-SecI recognition site | (Blank et al. 2011) |
pWRG99 | λ-Red genes under control of arabinose inducible promoter (P BAD ), I-SecI endonuclease under control of tetracycline inducible promoter (P tetA ), temperature sensitive, AmpR | (Blank et al. 2011) |
pMHM199 | I-SecI endonuclease under control of tetracycline inducible promoter (P tetA ), temperature sensitive, AmpR | This study |