Skip to main content

Table 1 Strains used in the study

From: Fast and efficient three-step target-specific curing of a virulence plasmid in Salmonella enterica

Strains Characteristic References
S. enterica sv. Typhimurium ATCC 14028 Wild type ATCC
S. enterica sv. Typhimurium MHM21 spvA::cat, I-SecI recognition site This study
S. enterica sv. Typhimurium MHM113 Lacks virulence plasmid p14028 This study
Plasmids
 pWRG100 cat, I-SecI recognition site (Blank et al. 2011)
 pWRG99 λ-Red genes under control of arabinose inducible promoter (P BAD ), I-SecI endonuclease under control of tetracycline inducible promoter (P tetA ), temperature sensitive, AmpR (Blank et al. 2011)
 pMHM199 I-SecI endonuclease under control of tetracycline inducible promoter (P tetA ), temperature sensitive, AmpR This study