Characterization of purified L36N trimerbodies. a Reducing SDS-PAGE of L36N trimerbody purified from HEK-293 cells or E. coli BL21(DE3) cells. b, c Size exclusion chromatogram profile, as measured by UV absorbance at 280 nm (blue trace) and molar mass (red trace), as measured by MALLS (only the data at the central part of the peak is shown, and can be read at the right hand axis). The mass measured at the center of the peak is indicated. d The functionality of purified L36N trimerbodies was demonstrated by direct ELISA with purified LM-111. Bound L36N was detected using HRP-conjugated protein A. e Serum stability. Purified L36 N trimerbodies were incubated at 37°C for different time periods in human serum. The antigen-binding activity was analyzed by ELISA against LM-111. The experiments were performed there times, and the mean values ± standard deviations are presented.