Fig. 1
From: Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies
a Schematic diagram of the scFv and the scFv-based N-terminal trimerbody gene constructs. Both constructs carry a N-terminal pelB signal sequence for potential periplasmic localization, the anti-laminin L36 scFv gene (VH and VL domains joined by a flexible linker), and C-terminal tags for immunodetection and purification (yellow box). The trimerbody gene constructs contains a TIEXVIII domain. b Illustration showing the arrangement of VH and VL domains in monomeric scFv and of VH, VL and TIEXVIII domains in the trimerbody.