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Table 4 Distribution of the 45 isolates re-tested using the CDT (43 with a ND and 2 with discrepant results between E-test and DNA-based assay)

From: A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae

 

Phenotypic test

DNA-based test

n

E-test

CD

CTX-M

TEM

SHV

Interpretation

E. cloacae, n = 6

ND

ESBL

Absent

Wild-type

Mutated

ESBL

1

ESBL

Present

Wild-type

Absent

ESBL

4

Non-ESBL (MMA55)*

ESBL*

Absent

Wild-type

Mutated

ESBL*

1*

E. coli, n = 20

ND

ESBL

Present

Wild-type

Absent

ESBL

16

Non-ESBL*

Present

Wild-type

Absent

ESBL*

4*

K. pneumoniae, n = 13

ND

Non-ESBL

Absent

Absent

Absent

Non-ESBL

2

ESBL

Absent

Wild-type

Mutated

ESBL

3

Present

Wild-type

Absent

ESBL

1

 

Wild-type

ESBL

5

 

Mutated

ESBL

1

ESBL (R080)*

ESBL*

Absent

Absent

Absent

Non-ESBL*

1*

Klebsiella spp., n = 1

ND

ESBL

Present

Wild-type

Absent

ESBL

1

P. mirabilis, n = 4

ND

ESBL

Absent

Wild-type

Mutated

ESBL

3

Non-ESBL*

Present

Absent

Absent

ESBL*

1*

S. marcescens, n = 1

ND

ESBL

Present

Wild-type

Absent

ESBL

1

  1. * Isolates for which genotypic and phenotypic results are discordant. Both discordant isolates and isolates for which E-test yielded a ‘ND’ result (n = 43) were re-tested using the combination disk test (CDT).