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AMB Express

Table 4 Distribution of the 45 isolates re-tested using the CDT (43 with a ND and 2 with discrepant results between E-test and DNA-based assay)

From: A qPCR and multiplex pyrosequencing assay combined with automated data processing for rapid and unambiguous detection of ESBL-producers Enterobacteriaceae

  Phenotypic test DNA-based test n
E-test CD CTX-M TEM SHV Interpretation
E. cloacae, n = 6 ND ESBL Absent Wild-type Mutated ESBL 1
ESBL Present Wild-type Absent ESBL 4
Non-ESBL (MMA55)* ESBL* Absent Wild-type Mutated ESBL* 1*
E. coli, n = 20 ND ESBL Present Wild-type Absent ESBL 16
Non-ESBL* Present Wild-type Absent ESBL* 4*
K. pneumoniae, n = 13 ND Non-ESBL Absent Absent Absent Non-ESBL 2
ESBL Absent Wild-type Mutated ESBL 3
Present Wild-type Absent ESBL 1
  Wild-type ESBL 5
  Mutated ESBL 1
ESBL (R080)* ESBL* Absent Absent Absent Non-ESBL* 1*
Klebsiella spp., n = 1 ND ESBL Present Wild-type Absent ESBL 1
P. mirabilis, n = 4 ND ESBL Absent Wild-type Mutated ESBL 3
Non-ESBL* Present Absent Absent ESBL* 1*
S. marcescens, n = 1 ND ESBL Present Wild-type Absent ESBL 1
  1. * Isolates for which genotypic and phenotypic results are discordant. Both discordant isolates and isolates for which E-test yielded a ‘ND’ result (n = 43) were re-tested using the combination disk test (CDT).
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