Fig. 1

Output illustration of the web interactive application. For a given isolate, integration of PCR and multiplex pyrosequencing results allows the determination of the ESBL-producing status (available at https://ucl-irec-ctma.shinyapps.io/Pyrosequencing-TEM-SHV/). As demonstrated in this example, the algorithm enables to correctly decompose the multiplex pyrosequencing signal generated for the blaSHV gene despite the presence of two sequences for the blaSHV238-240 genomic region (i.e., G238 and E240 on one variant and the G238S-E240K double substitutions on another one).