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Table 1 PCR primers used for amplification of vgb and the amo1 promoter

From: Engineering of Nitrosomonas europaea to express Vitreoscilla hemoglobin enhances oxygen uptake and conversion of ammonia to nitrite

Primer Sequence (5′–3′) Annealing temp (°C)
Vgb1 GCG CGG AAT TCA TGT TAG ACC AGC AA 59
Vgb2 GCG CGC TCG AGT TAT TCA ACC GCT TG 59 (60)*
AmoCp1 AGC TAG CTT GAC TAA AAA GGT CAA GGT GAC TAG CCT TTC AGA TGT 62 (60)*
  1. * Annealing temperature used for the primer pair.