Skip to main content

Table 1 PCR primers used for amplification of vgb and the amo1 promoter

From: Engineering of Nitrosomonas europaea to express Vitreoscilla hemoglobin enhances oxygen uptake and conversion of ammonia to nitrite

Primer

Sequence (5′–3′)

Annealing temp (°C)

Vgb1

GCG CGG AAT TCA TGT TAG ACC AGC AA

59

Vgb2

GCG CGC TCG AGT TAT TCA ACC GCT TG

59 (60)*

AmoCp1

AGC TAG CTT GAC TAA AAA GGT CAA GGT GAC TAG CCT TTC AGA TGT

62 (60)*

  1. * Annealing temperature used for the primer pair.