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Table 2 PCR primers for genes that confer resistance to arsenic

From: Community of thermoacidophilic and arsenic resistant microorganisms isolated from a deep profile of mine heaps

Name

Sequence 5′–3′

Gene

References

Primer #1F

5′-GTSGGBTGYGGMTAYCABGYCTA-3′

aioB (formerly aoxB)

Inskeep et al. (2007)

Primer #1R

5′-TTGTASGCBGGNCGRTTRTGRAT-3′

darsB1F

5′-GGTGTGGAACATCGTCTGGAAYGCNAC-3′

arsB

Achour et al. (2007)

darsB1R

5′-CAGGCCGTACACCACCAGRTACATNCC-3′

dacr1F

5′-GCCATCGGCCTGATCGTNATGATGTAYCC-3′

arr3 (formerly ACR3 1)

dacr1R

5′-CGGCGATGGCCAGCTCYAAYTTYTT-3′

dacr5F

5′-TGATCTGGGTCATGATCTTCCCVATGMTGVT-3′

arr3 (formerly ACR3 2)

dacr4R

5′-CGGCCACGGCCAGYTCRAARAARTT-3′

  1. aio arsenite oxidase, ars arsenic resistance system, arr arsenate respiratory-reduction.