Neighbor-joining tree showing phylogenetic relationships among different microorganisms isolated from mine heaps, based on partial 16S rDNA sequences. Node numbers are support based on 500 bootstrap replicates. Representative 16S rDNA clones used for the analysis were: T54-17; Q18-12; T54-2; T27-14; Q18-9; Q63-13 and Q18-15. Sequences from NCBI GenBank used for the analysis were: Pseudomonas mendocina HQ113219.1; Comamonadaceae bacterium AY651926.1; Paenibacillus pasadenensis AY167820.1; Acidobacteria bacterium GU187033.1; Sulfobacillus thermosulfidooxidans GU180244.1; Uncultured Sulfobacillus EF612378.1; Sulfobacillus acidophillus AY140234.1; Sulfobacillus sp. DQ350778.1; Escherichia coli AB599716.1 was used as the out-group.