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Fig. 5 | AMB Express

Fig. 5

From: A lipophilic fluorescent LipidGreen1-based quantification method for high-throughput screening analysis of intracellular poly-3-hydroxybutyrate

Fig. 5

Fluorescence intensities of the mutant library, which consisted of a pool of phaC genes with PCR-introduced random mutations. Every single mutant clone was inoculated in a 96-deep well plate and cultivated for 20 h in 800 µl of LB medium with 20 g/L glucose. Fluorescence intensities were monitored after transferring 100 µl of culture solution into a black microplate. The x axis represents the names of the mutants.

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