Fluorescence of PHB-producing cells grown on a LipidGreen-containing agar plate. a Fluorescence staining of PHB-producing and PHB-nonproducing cells on an agar plate. E. coli harboring pPhaCAB (left side of the plate) had bright fluorescence under UV light (302 nm) in contrast to E. coli containing pBluescript II SK+ vector (right side of the plate) exhibiting a much weaker fluorescence than above. b Microscopic observation of E. coli stained by LipidGreen1 on agar plates. The cell suspension in PBS buffer was placed on a glass slide to obtain optic (left) and fluorescent (right) images. The images were viewed under the fluorescence microscope (×1,000 amplification) with a green fluorescence filter.