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Table 1 Strain and plasmids used in this work

From: Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities

Strain and plasmids

Genotype

Reference

Strains

 E coli

  XL1-Blue

EndA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F’[::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(r K  m +K )

Stratagene

 P. pastoris

  X33

Wild type

 

  X33/pPICZαA-clavMO

X33 transformed with the plasmid pPICZαA-clavMO

This work

  X33/pGAPZαB-clavMO

X33 transformed with the plasmid pGAPZαB-clavMO

This work

Plasmids

 pPICZα A

Fator-α as secretion signal, AOX1 promotor, bler

 

 pGAPZα B

Fator-α as secretion signal, PGAP promotor, bler

 

 pPICZαA-clavMO

pPICZαA with the gene coding for the cassette thioredoxin-clavanin MO

This work

 pGAPZαB-clavMO

pGAPZαB with the gene coding for the cassette thioredoxin-clavanin MO

This work