|
Strains
|
|
E coli
|
|
XL1-Blue
|
EndA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F’[::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(r
−K
m
+K
)
|
Stratagene
|
|
P. pastoris
|
|
X33
|
Wild type
| |
|
X33/pPICZαA-clavMO
|
X33 transformed with the plasmid pPICZαA-clavMO
|
This work
|
|
X33/pGAPZαB-clavMO
|
X33 transformed with the plasmid pGAPZαB-clavMO
|
This work
|
|
Plasmids
|
|
pPICZα A
|
Fator-α as secretion signal, AOX1 promotor, bler
| |
|
pGAPZα B
|
Fator-α as secretion signal, PGAP promotor, bler
| |
|
pPICZαA-clavMO
|
pPICZαA with the gene coding for the cassette thioredoxin-clavanin MO
|
This work
|
|
pGAPZαB-clavMO
|
pGAPZαB with the gene coding for the cassette thioredoxin-clavanin MO
|
This work
|
