Strain and plasmids | Genotype | Reference |
---|---|---|
Strains | ||
E coli | ||
XL1-Blue | EndA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F’[::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(r −K m +K ) | Stratagene |
P. pastoris | ||
X33 | Wild type | |
X33/pPICZαA-clavMO | X33 transformed with the plasmid pPICZαA-clavMO | This work |
X33/pGAPZαB-clavMO | X33 transformed with the plasmid pGAPZαB-clavMO | This work |
Plasmids | ||
pPICZα A | Fator-α as secretion signal, AOX1 promotor, bler | |
pGAPZα B | Fator-α as secretion signal, PGAP promotor, bler | |
pPICZαA-clavMO | pPICZαA with the gene coding for the cassette thioredoxin-clavanin MO | This work |
pGAPZαB-clavMO | pGAPZαB with the gene coding for the cassette thioredoxin-clavanin MO | This work |