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Table 1 Strain and plasmids used in this work

From: Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities

Strain and plasmids Genotype Reference
Strains
 E coli
  XL1-Blue EndA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F’[::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(r K  m +K ) Stratagene
 P. pastoris
  X33 Wild type  
  X33/pPICZαA-clavMO X33 transformed with the plasmid pPICZαA-clavMO This work
  X33/pGAPZαB-clavMO X33 transformed with the plasmid pGAPZαB-clavMO This work
Plasmids
 pPICZα A Fator-α as secretion signal, AOX1 promotor, bler  
 pGAPZα B Fator-α as secretion signal, PGAP promotor, bler  
 pPICZαA-clavMO pPICZαA with the gene coding for the cassette thioredoxin-clavanin MO This work
 pGAPZαB-clavMO pGAPZαB with the gene coding for the cassette thioredoxin-clavanin MO This work