Identification of genes coding for putative wax ester synthase/diacylglycerol acyltransferase enzymes in terrestrial and marine environments

AMB Express

Table 1 Primer pairs to amplify genes encoding WS/DGAT used in this study

Primer	Sequence (5′-3′)	Cycling conditions (°C s⁻¹)	Specificity
TgsXup	CGCCCGCTSTGGGAGATG	94/300, (94/30, 58/30,72/60) ×30, 72/300	Actinobacteria/
TgsXdown	CGGGCCSGSGACGTTSGA	94/300, (94/30, 58/30,72/60) ×30, 72/300	γ-Proteobacteria
Gram—F	CARCCYATGCAYGTWGG	94/300, (94/30, 45/45, 72/45) ×30, 72/300	Gram negative bacteria
Gram—R	ATMAYCASRTTGAAGGC	94/300, (94/30, 45/45, 72/45) ×30, 72/300	Gram negative bacteria
RhodoF	CCGATGTGGGARMTGCAC	94/300, (94/30, 48/45, 72/45) ×30, 72/300	Rhodococcus
RhodoR	ATSATCACGTTGAACGGC	94/300, (94/30, 48/45, 72/45) ×30, 72/300	Rhodococcus
Maratf1	ACGCCCCTGAATCCCACT	94/500, (94/60, 54/60, 72/90) ×30, 72/600	Marinobacter
Mar130F	TGTCATGTTCAGGGCCAGYC	94/500, (94/60, 54/60, 72/90) ×30, 72/600	Marinobacter