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Table 1 Primer pairs to amplify genes encoding WS/DGAT used in this study

From: Identification of genes coding for putative wax ester synthase/diacylglycerol acyltransferase enzymes in terrestrial and marine environments

Primer Sequence (5′-3′) Cycling conditions (°C s−1) Specificity
TgsXup CGCCCGCTSTGGGAGATG 94/300, (94/30, 58/30,72/60) ×30, 72/300 Actinobacteria/
TgsXdown CGGGCCSGSGACGTTSGA γ-Proteobacteria
Gram—F CARCCYATGCAYGTWGG 94/300, (94/30, 45/45, 72/45) ×30, 72/300 Gram negative bacteria
Gram—R ATMAYCASRTTGAAGGC
RhodoF CCGATGTGGGARMTGCAC 94/300, (94/30, 48/45, 72/45) ×30, 72/300 Rhodococcus
RhodoR ATSATCACGTTGAACGGC
Maratf1 ACGCCCCTGAATCCCACT 94/500, (94/60, 54/60, 72/90) ×30, 72/600 Marinobacter
Mar130F TGTCATGTTCAGGGCCAGYC