Table 1 Plasmids used in this study
Plasmid | Characteristics | References |
|---|---|---|
pQE-30-LE | HIS-STREP, separated by LguI/Eco81I overlapping restriction site | Marquardt et al. (2014) |
pQE-30-LE::cel5A | HIS-Cel5A-STREP | Marquardt et al. (2014) |
pQE-80L::bgl1 | HIS-Bgl1 | Schröder et al. (2014) |
pQE-30-LE::bgl1 | HIS-Bgl1-STREP | This study |
pQE-30-LE::CB | HIS-Cel5A-Bgl1-STREP | This study |
pQE-30-LE::BC | HIS-Bgl1-Cel5A-STREP | This study |
pQE-30-LE::CE294AB | HIS-Cel5AE294A-Bgl1-STREP, inactivated endoglucanase | This study |
pQE-30-LE::BCE294A | HIS-Bgl1-Cel5AE294A-STREP, inactivated endoglucanase | This study |
pQE-80L::bgl1_G395 | HIS-Bgl1E395G, inactivated β-glucosidase | Schröder et al. (2014) |
pQE-30-LE::CBE395G | HIS-Cel5A-Bgl1E395G-STREP, inactivated β-glucosidase | This study |
pQE-30-LE::BE395GC | HIS-Bgl1E395G-Cel5A-STREP, inactivated β-glucosidase | This study |