Influence of pH on catalytic activity of fusion constructs compared to single proteins. a Experiments were done with β-glucan as substrate at different pH and constant temperature of 80°C. b Enzymatic activity of β-glucosidases using 4-NP-β-d-GP as substrate. Experiments were done using Cel5A (filled diamonds, dashed line), Bgl1 (filled circles, dashed line), CB (filled squares, continuous line) and BC (filled triangles, continuous line). All experiments were done in triplicate.