Figure 4From: Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimerasInfluence of temperature on fusion and single constructs. a Purified proteins were tested using β-glucan as substrate at different temperatures and constant of pH 6. b Measurement of enzymatic activity towards 4-NP-β-d-GP. Experiments were done using Cel5A (filled diamonds, dashed line), Bgl1 (filled circles, dashed line), CB (filled squares, continuous line) and BC (filled triangles, continuous line). All experiments were done in triplicate.Back to article page