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AMB Express
Figure 2 | AMB Express

Figure 2

From: Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras

Figure 2

Purification of double-tagged fusion constructs produced in E. coli. a Purification of Cel5A-Bgl1 fusion construct. SDS-PAGE analysis and western blot of crude cell extracts and purification steps. b Visualization of Bgl1-Cel5A fusion protein by SDS-PAGE and western blotting analyses. M molecular weight marker, −IPTG total cellular protein, no induction, +IPTG crude extracts, 1 mM IPTG, Pe pellet fraction, insoluble cell debris, SN supernatant, soluble proteins, HP heat precipitation, heat stable proteins, NiNTA affinity chromatography step 1, Strep affinity chromatography step 2. Molecular weights are indicated aside.

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