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Table 1 Bacterial strains and plasmids used in this study

From: Engineering alternative isobutanol production platforms

Strains Genotype References
Escherichia coli DH10B F, mcrA, Δ(mrr-hsdRMS-mcrBC), f80ΔlacZDM15 ΔlacX74, deoR, recA1, endA1, araD139, Δ(ara,leu)7697, galU, galK, rpsL, nupG, λ Invitrogen
Escherichia coli W Type strain. Waksman’s strain ATCC9637
Shimwellia blattae Type strain CIP 104942, DSMZ 4481
Plasmids
 pUC57 ApR, pUC-derived cloning vector for E. coli bearing Plac. rep (pMB1) Genscript
 pIZ1016 GmR, broad-host range expression vector bearing lacIq and Ptac. rep (pBBR1MCS) Martínez-Perez et al. (2004)
 pSEVA424 SmR/SpR, broad-host range expression vector bearing lacIq and Ptrc.rep (RK2 replicative origin. oriV-trfA) Silva-Rocha et al. (2013)
 pUC57-IbPSO ApR, IbPSO into pUC57 This work
 pIZIbPSO GmR, IbPSO into pIZ1016 This work
 p424IbPSO SmR/SpR, IbPSO into pSEVA424 This work