Schematic representation of the genes contained in the IbPSO synthetic operon. The sequences of the intergenic regions (R1–R5) are indicated in the table. The sequences of the restriction sites are underlined and the corresponding restriction enzymes are annotated. The RBS sequences (AGGAGG) are indicated in red. The maps of the two broad host range plasmids, pSEVA424 and pIZ1016, used to clone the IbPSO operon are shown.