Figure 3

Phylogenetic tree of archaeal 16S rRNA gene sequences from methanogenic alkanes-degrading enrichment culture (in red) and rooted with outgroup sequence from Escherichia coli (J01695). The OTUs are shown with clone names and accession numbers. Sequences from oil or hydrocarbon-impacted environments are in black bold. The topology of the tree was obtained by the neighbor-joining method. Bootstrap values (n = 1,000 replicates), the values below 75% are not shown (a); phylogenetic tree of deduced amino acid sequences of methyl coenzyme-M reductase genes (mcrA) from methanogenic alkanes-degrading enrichment culture (in red). Topology of the tree was obtained by the neighbor-joining method. The evolutionary distances were computed using the Poisson correction method. Bootstrap values (n = 1,000 replicates), values below 75% are not shown (b).