Figure 6From: Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysisProposed substrate recognition systems of hemicellulose and pectin in C. cellulovorans. For hemicellulose, polysaccharides are degraded to derived oligosaccharides outside of the cell, and extracellular solute-binding proteins bind these substrates. Solute-binding proteins induce signal-to-signal transduction integral membrane histidine kinases. Next the activated kinases phosphorylate transcriptional regulator AraC, and target genes (shown in Figure 5) are upregulated (a). For pectin, polysaccharides are de-esterified by pectinesterase and degraded by pectin lyase. Next, derived oligosaccharides are transported into cell, and these or other metabolites bind to a transcriptional regulator. Then, genes belonging to the target cluster (shown in Figure 5) are upregulated (b).Back to article page