24-h old Salmonella biofilm dispersal by MAHMA NONOate dissolved in BPS and CNC. Biofilms were preformed by S. enterica typhimurium 14028 for 24 h on polystyrene prior to the treatment with MAHMA NONOate and molsidomine. Contact time at which biofilms were exposed to the nitric oxide donor is listed on each panel. a, b The decreasing of biofilm formation was measured by using the technique of crystal violet staining. c, d Fluorescent Salmonella 14028 pGFP-ON biofilm was treated with MAHMA NONOate and molsidomine dissolved in CNC. Increase in fluorescence of the planktonic cells was measured at 485 nm/535 nm. The box-plots encompass the lower and upper quartiles, thick lines within the box are the median values, and the whiskers indicate the degree of dispersion of the data. Outliers are shown as dots. Dotted line represents the grand mean. Letters above each box represent the Tukey means separation: different letters are significant different means.