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Figure 4 | AMB Express

Figure 4

From: Functional screening system for yeast-secreted peptides acting on G-protein coupled receptors

Figure 4

Direct activity detection of various yeast-secreted GLP1R agonists. a Schematic illustration for direct activity detection assay was depicted. Single colony of yeasts was inoculated in 96-well plate containing SDC and cultivated for 48 h. After removal of SDC, DMEM was added and incubated for additional 12 h. Then, the culture was subjected to GLP1R activation assay using GLP1R-CHO. b Seven kinds of yeasts were tested for GLP1R activation potency according to the assay flow mentioned above. The data represent the average ± SEM of three independent experiments.

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