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Figure 3 | AMB Express

Figure 3

From: Functional screening system for yeast-secreted peptides acting on G-protein coupled receptors

Figure 3

Scale-down assay for GLP1R activation by yeast-secreted peptides. a Indicated number of yeast cells, Ctrl-yeast (left bar) or Ex4-yeast (right bar), were seeded in a 96-well plate containing DMEM and incubated for 12 h. Then, GLP1R activation assay was conducted to find the yeast cell number providing wider dynamic range. The data represent the average ± SEM of three independent experiments. b Yeast cultivation time to achieve the target yeast cell number, 1 × 106, was evaluated. Yeasts were seeded in a 96-well plate containing SDC at the initial number of 3. After 24-, 48-, and 72-h cultivations, the number of yeasts was determined using hematometer. The data represent the average ± SEM of three independent experiments.

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