|
Reductase-strain
|
Substrate changed*
|
Maximum activity (U mg−1)
|
k
cat (s−1)
|
K
M (µM)
|
Catalytic efficiency (s−1 µM−1)
|
|---|
|
StyB-1CP
|
NADH
|
75.1 ± 1.8
|
26.9 ± 0.6
|
59.3 ± 4.2
|
0.454
|
|
FAD
|
91.9 ± 6.6
|
32.9 ± 2.3
|
108.3 ± 18.3
|
0.304
|
|
StyB-ST
|
NADH
|
28.8
|
8.8 ± 0.1
|
18.9 ± 1.0
|
0.466
|
|
FAD
|
28.7
|
8.7 ± 0.3
|
2.6 ± 0.4
|
3.346
|
|
StyA2B-1CP**
|
NADH
|
3.7
|
3.9 ± 0.3
|
58 ± 9
|
0.068
|
|
FAD
|
4.9
|
5.2 ± 0.2
|
26 ± 2
|
0.203
|
- * One of the substrates was applied in excess and the concentration of the other one was varied.
- ** Data as previously reported (Tischler et al. 2010).
