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Table 1 Plasmids and primers used in this study

From: Catalytic and hydrodynamic properties of styrene monooxygenases from Rhodococcus opacus 1CP are modulated by cofactor binding

Plasmid or primer Relevant characteristic(s) Source
Plasmids
 pSRoA_P01 styA of R. opacus 1CP (1.281-kb NdeI/KpnI fragment) cloned into pET16bP This study
 pSRoB_P01 styB of R. opacus 1CP (545-kb NdeI/NotI fragment) cloned into pET16bP This study
 pSPfB_P01 styB of Pseudomonas fluorescens ST (510-kb KpnI fragment) cloned into pET16bp This study
 pJET1.2/blunt Contains a gene coding for a lethal restriction enzyme; a gene disrupted by ligation of a DNA insert into the cloning site is possible; T7 promoter Thermo Scientific
 pET16bp pET16b with additional multicloning site; allows expression of recombinant proteins with N-terminal His10-tag U. Wehmeyer*
 pEX_A_StyB pEX vector with additional multiple cloning site; (Ampr) Eurofins MWG Operon
Primers
 1CP_A_fw 5′-CATATGAGCAAGCGAATC-3′; includes NdeI site This study
 1CP_A_rev 5′-GGTACCTCATGGCTGTGC-3′; includes KpnI site This study
 ST_B_fw 5′-GGTACCATATGACGTTAAAAAAAGATGTG-3′; includes KpnI site This study
 ST_B_rev 5′-GGTACCTTAGTTCAGCGGCAACGGCTT G-3′; includes KpnI site This study
  1. * Personal communication; Tischler et al. (2009).