Table 1 Plasmids and primers used in this study
Plasmid or primer | Relevant characteristic(s) | Source |
|---|---|---|
Plasmids | ||
 pSRoA_P01 | styA of R. opacus 1CP (1.281-kb NdeI/KpnI fragment) cloned into pET16bP | This study |
 pSRoB_P01 | styB of R. opacus 1CP (545-kb NdeI/NotI fragment) cloned into pET16bP | This study |
 pSPfB_P01 | styB of Pseudomonas fluorescens ST (510-kb KpnI fragment) cloned into pET16bp | This study |
 pJET1.2/blunt | Contains a gene coding for a lethal restriction enzyme; a gene disrupted by ligation of a DNA insert into the cloning site is possible; T7 promoter | Thermo Scientific |
 pET16bp | pET16b with additional multicloning site; allows expression of recombinant proteins with N-terminal His10-tag | U. Wehmeyer* |
 pEX_A_StyB | pEX vector with additional multiple cloning site; (Ampr) | Eurofins MWG Operon |
Primers | ||
 1CP_A_fw | 5′-CATATGAGCAAGCGAATC-3′; includes NdeI site | This study |
 1CP_A_rev | 5′-GGTACCTCATGGCTGTGC-3′; includes KpnI site | This study |
 ST_B_fw | 5′-GGTACCATATGACGTTAAAAAAAGATGTG-3′; includes KpnI site | This study |
 ST_B_rev | 5′-GGTACCTTAGTTCAGCGGCAACGGCTT G-3′; includes KpnI site | This study |