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AMB Express

Table 2 Counting of B. pumilus SAFR-032 spores (in CFU mL -1 )

From: Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide

Exposure agent

CFU mL -1 , 1 h exposure

CFU mL -1 , 24 h exposure

Nothing (control)

9.4 × 109

2.65 × 109

Peroxide, 3.5% v/v solution

1.47 × 108

<5 × 102

Inactivated ClO2 solution

2.79 × 108

6.3 × 109

Activated ClO2 solution

1.6 × 105

<1 × 104

  1. Notes: Results of duplicate plate-counting experiments using 100 μL aliquots of exposed spores on aluminum coupons in serial dilutions of PBS following ultrasonication into sodium thiosulfate to inactivate chlorine dioxide. Inactive ClO2 concentration = 108.5 μg/mL total ClO2; active ClO2 concentration = 70.4 μg/mL total ClO2, 39 μg/mL free ClO2. CFU mL-1 values based on the first plates in the serial dilution to not be too numerous to count (>300 total CFU or >100 CFU in one quarter of plate).
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