Yeast extract (g/L)
|
Cell density (g
CDW
/L)
|
PHA (g
PHA
/L)
|
PHA content (wt%)
|
Cultivation time (h)
|
---|
0.2 | 14.6 | 5.58 | 38.3 | 48 |
0.67 | 27.3 | 14.2 | 48.1 | 48 |
6.7 | 44.6 | 21.1 | 47.2 | 48 |
- During cultivation, glycerol was intermittently added. Kanamycin was added as 50 μg/ml final concentration at the beginning of the cultivation. All cultivations were conducted in a 2 L jar-fermenter.