Strain and process development for poly(3HB-co-3HP) fermentation by engineered Shimwellia blattae from glycerol

AMB Express

Table 2 Results of fed-batch cultivation using Sb 6BP and glycerol as sole carbon source

Strain	Medium	Condition	Cell density (g _CDW /L)	PHA content (wt%)	PHA (g _PHA /L)	Monomer composition (mol%)		Cultivation time (h)	PHB(P) productivity (mg _PHA /L/h)	Glycerol yield
Strain	Medium	Condition	Cell density (g _CDW /L)	PHA content (wt%)	PHA (g _PHA /L)	3HB	3HP	Cultivation time (h)	PHB(P) productivity (mg _PHA /L/h)	Glycerol yield
Sb6BP	BM	Anaerobic	1.72	14.7	0.25	83.4	16.6	72	3.51	0.0041
		Low aeration/agitation	2.11	24.6	0.52	92.4	7.3		7.21	0.0075
		Two-step	6.12	29.9	1.83	91.4	8.6		25.4	0.032
		Aerobic	12.6	55.2	6.96	100	0		96.6	0.097
	MMB	Anaerobic	1.84	17.2	0.32	82.9	18.1	48	6.59	0.0033
		Low aeration/agitation	3.78	20.3	0.77	91.7	8.3		16.0	0.011
		Two-step	23.2	30.7	7.12	97.9	2.1		148.4	0.093
		Aerobic	27.3	48.1	13.1	100	0		273.6	0.15

Cells were cultivated in MB or MMB medium with glycerol that was intermittently added. MMB contained 0.67 g/L yeast extract. Kanamycin was added as 50 μg/ml final concentration at the beginning of the cultivation. All cultivations were conducted in a 2 L jar-fermenter.