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Table 2 Results of fed-batch cultivation using Sb 6BP and glycerol as sole carbon source

From: Strain and process development for poly(3HB-co-3HP) fermentation by engineered Shimwellia blattae from glycerol

Strain Medium Condition Cell density (g CDW /L) PHA content (wt%) PHA (g PHA /L) Monomer composition (mol%) Cultivation time (h) PHB(P) productivity (mg PHA /L/h) Glycerol yield
3HB 3HP
Sb6BP BM Anaerobic 1.72 14.7 0.25 83.4 16.6 72 3.51 0.0041
Low aeration/agitation 2.11 24.6 0.52 92.4 7.3 7.21 0.0075
Two-step 6.12 29.9 1.83 91.4 8.6 25.4 0.032
Aerobic 12.6 55.2 6.96 100 0 96.6 0.097
MMB Anaerobic 1.84 17.2 0.32 82.9 18.1 48 6.59 0.0033
Low aeration/agitation 3.78 20.3 0.77 91.7 8.3 16.0 0.011
Two-step 23.2 30.7 7.12 97.9 2.1 148.4 0.093
Aerobic 27.3 48.1 13.1 100 0 273.6 0.15
  1. Cells were cultivated in MB or MMB medium with glycerol that was intermittently added. MMB contained 0.67 g/L yeast extract. Kanamycin was added as 50 μg/ml final concentration at the beginning of the cultivation. All cultivations were conducted in a 2 L jar-fermenter.