|
Strain
|
Medium
|
Condition
|
Cell density (g
CDW
/L)
|
PHA content (wt%)
|
PHA (g
PHA
/L)
|
Monomer composition (mol%)
|
Cultivation time (h)
|
PHB(P) productivity (mg
PHA
/L/h)
|
Glycerol yield
|
|---|
|
3HB
|
3HP
|
|---|
|
Sb6BP
|
BM
|
Anaerobic
|
1.72
|
14.7
|
0.25
|
83.4
|
16.6
|
72
|
3.51
|
0.0041
|
|
Low aeration/agitation
|
2.11
|
24.6
|
0.52
|
92.4
|
7.3
|
7.21
|
0.0075
|
|
Two-step
|
6.12
|
29.9
|
1.83
|
91.4
|
8.6
|
25.4
|
0.032
|
|
Aerobic
|
12.6
|
55.2
|
6.96
|
100
|
0
|
96.6
|
0.097
|
|
MMB
|
Anaerobic
|
1.84
|
17.2
|
0.32
|
82.9
|
18.1
|
48
|
6.59
|
0.0033
|
|
Low aeration/agitation
|
3.78
|
20.3
|
0.77
|
91.7
|
8.3
|
16.0
|
0.011
|
|
Two-step
|
23.2
|
30.7
|
7.12
|
97.9
|
2.1
|
148.4
|
0.093
|
|
Aerobic
|
27.3
|
48.1
|
13.1
|
100
|
0
|
273.6
|
0.15
|
- Cells were cultivated in MB or MMB medium with glycerol that was intermittently added. MMB contained 0.67 g/L yeast extract. Kanamycin was added as 50 μg/ml final concentration at the beginning of the cultivation. All cultivations were conducted in a 2 L jar-fermenter.
