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Table 1 Bacterial strains and plasmids used in this study

From: Strain and process development for poly(3HB-co-3HP) fermentation by engineered Shimwellia blattae from glycerol

Strains and plasmids Relevant characteristics Origin or reference
Strains   
E. coli   
TOP10 F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 mupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1λ Life technologies (Darmstadt, D)
S. blattae   
ATCC33430 Wild type strain ATCC33430
Sb6BP pBBR1MCS-2 ::plac::aldD::dhaT:: pct::plac::phaC1AB in S. blattae ATCC33430 This study
Plasmids   
pBBR1MCS-2 Cloning vector, Kmr Kovach et al., 1995
pBBR1MCS-2 ::aldD::dhaT::pct Kmr; aldD Pp ; dhaT Pp ; pct Cp Heinrich et al., 2013
pBBR1MCS-2 ::plac::phaC1AB Kmr; phaC1 Re ; phaA Re ; phaB1 Re This study
pBBR1MCS-2 ::plac::aldD::dhaT::pct ::plac::phaC1AB Kmr; aldD Pp ; dhaT Pp ; pct Cp ; phaC1 Re ; phaA Re ; phaB1 Re This study