Table 1 Bacterial strains and plasmids used in this study
Strains and plasmids | Relevant characteristics | Origin or reference |
|---|---|---|
Strains | ||
E. coli | ||
TOP10 | F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 mupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1λ | Life technologies (Darmstadt, D) |
S. blattae | ||
ATCC33430 | Wild type strain | ATCC33430 |
Sb6BP | pBBR1MCS-2 ::plac::aldD::dhaT:: pct::plac::phaC1AB in S. blattae ATCC33430 | This study |
Plasmids | ||
pBBR1MCS-2 | Cloning vector, Kmr | Kovach et al., 1995 |
pBBR1MCS-2 ::aldD::dhaT::pct | Kmr; aldD Pp ; dhaT Pp ; pct Cp | Heinrich et al., 2013 |
pBBR1MCS-2 ::plac::phaC1AB | Kmr; phaC1 Re ; phaA Re ; phaB1 Re | This study |
pBBR1MCS-2 ::plac::aldD::dhaT::pct ::plac::phaC1AB | Kmr; aldD Pp ; dhaT Pp ; pct Cp ; phaC1 Re ; phaA Re ; phaB1 Re | This study |