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Table 2 Substrate specificity

From: Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase

Substrate

Relative activity (%) a

Isovaleraldehyde

244 ± 1.9

Methylglyoxal

100

Valeraldehyde

95.6 ± 1.6

Hexanal

81.4 ± 1.4

Heptanal

80.8 ± 2.5

Furfural

60.1 ± 1.7

Propionaldehyde

49.3 ± 0.7

Octanal

22.3 ± 1.5

Benzaldehyde

14.3 ± 2.0

HMF

1.0

Cinnamaldehyde

N/Ab

Vanillin

N/Ab

  1. aReductive activities were measured in 100 mM acetate buffer (pH 5.5) containing 3 mM substrate, 0.1 mM NADPH and 100 nM enzyme.
  2. bN/A means no measurable activity. Due to the high absorbance of this substrate at 340 nm, activity was not determined under the assay conditions.