Substrate
|
Relative activity (%)
a
|
---|
Isovaleraldehyde
|
244 ± 1.9
|
Methylglyoxal
|
100
|
Valeraldehyde
|
95.6 ± 1.6
|
Hexanal
|
81.4 ± 1.4
|
Heptanal
|
80.8 ± 2.5
|
Furfural
|
60.1 ± 1.7
|
Propionaldehyde
|
49.3 ± 0.7
|
Octanal
|
22.3 ± 1.5
|
Benzaldehyde
|
14.3 ± 2.0
|
HMF
| 1.0≧ |
Cinnamaldehyde
|
N/Ab
|
Vanillin
|
N/Ab
|
-
aReductive activities were measured in 100 mM acetate buffer (pH 5.5) containing 3 mM substrate, 0.1 mM NADPH and 100 nM enzyme.
-
bN/A means no measurable activity. Due to the high absorbance of this substrate at 340 nm, activity was not determined under the assay conditions.