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Table 1 Purification of KmGRE2 from E. coli BL21 (DE3)

From: Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase

Purification step Total protein (mg) Total activity (U) Specific activity (U/mg) Yield (%)
Crude extract 215 410 1.91 100
Chelating sepharose fast flow column 79.1 371 4.70 90.5
HiLoad 26/60 superdex 200 pg column 40.0 338 8.45 82.4