Table 1 Purification of KmGRE2 from E. coli BL21 (DE3)
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|
Crude extract | 215 | 410 | 1.91 | 100 |
Chelating sepharose fast flow column | 79.1 | 371 | 4.70 | 90.5 |
HiLoad 26/60 superdex 200 pg column | 40.0 | 338 | 8.45 | 82.4 |