Effects of pH and temperature on KmGRE2 activity and stability. The markers of buffer were indicated following: circles, acetate; squares, citrate; diamonds, phosphate, isosceles triangles, borate-NaOH and right triangles, bicarbonate-NaOH. (A) Effect of pH on KmGRE2 activity. (B) Effect of temperature on KmGRE2 activity. (C) KmGRE2 activity after incubation for 30 min at various temperatures in the 20 mM Tris–HCl buffer (pH 7.2). (D) KmGRE2 activity after incubation for 30 min at 35°C in buffer solutions of various pHs. Error bars indicate SE (n = 3).