Sugar consumption and ethanol production by
S. cerevisiae. Cells of un-evolved (A) and evolved strains (B) were grown in YNB-medium supplemented with 4% glucose until mid-log phase, collected by centrifugation, washed with distilled water and transferred to fermentation medium containing 3% glucose and 1% xylose. The samples were collected in times of 0, 6, 20, 24, 28, 44, 48 hours and the supernatants were used to determine the concentration of glucose and xylose, in times of 24 and 48 hours the supernatants were used to determine the ethanol concentration. The results represent the mean ± standard deviation of at least three independent experiments.