Measurement of extracellular endo-glucanase activity. A) Enzyme activity tested through hydrolysis of CMC by supernatants of bacteria grown on CMC and bagasse. Cell and fiber-free supernatants were incubated for 10 days at 30°C before staining with Congo red. Halos around wells indicate extracellular endo-glucanase activity. B) Total extracellular protein concentration as measured by the method of Bradford of bacterial cultures incubated in medium containing sugarcane residues or CMC. C) CMCase activity in supernatants of bacterial strain selected for high cellulolytic activity grown on CMC and bagasse expressed in units.ml-1. Bacterial abbreviations: E = Enterococcus casseliflavus Kd7 TUC-EEAOC, K = Klebsiella oxytoca Kd70 TUC-EEAOC and B = Bacillus pumilus Kd109 TUC-EEAOC, S = Stenotrophomonas maltophilia Kd3 TUC-EEAOC.