From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose
Strain | Genotype | Carbon source | Growth conditions | PHB yield/carbon source | PHB content (mg/g CDW) | Reference | |
---|---|---|---|---|---|---|---|
(mg/g) | (Cmmol/Cmol) | ||||||
SCKK005 | PHB pathway | Glucose | Shake flask | ~1.5a | 2.1a | 4.02 | (Kocharin et al. 2013) |
SCK006 | PHB pathway, ADH2, ALD6, acs L641P, ERG10 | Glucose | Shake flask | ~5a | 7a | 15.89 | (Kocharin et al. 2013) |
SCK032 | PHB pathway, xpkA, ack, acs L641P | Glucose | Shake flask | ~9a | 12.5a | 27.86 | (Kocharin et al. 2013) |
SCK034 | PHB pathway, gapN, ADH2, ALD6, acs L641P, ERG10 | Glucose | Shake flask | ~7a | 9.8a | 27.52 | (Kocharin et al. 2013) |
SCKK006 | PHB pathway, ADH2, ALD6, acs L641P, ERG10 | Glucose | Aerobic, chemostat bioreactor, dilution rate 0.10 h−1 | 2.79 | 3.67 | 5.59 | (Kocharin and Nielsen 2013) |
Strain RKS | GAL1-10-induced PHB pathway | Galactose/Glucose | Aerobic, batch bioreactor | 13.5b | 18.8b | 75 | (Carlson and Srienc 2006) |
TMB4443 | PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ | Xylose | Shake flask | 1.17 | 1.63 | 10.4 | This study |
TMB4443 | PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ | Xylose | Aerobic, batch bioreactor | 1.99 | 2.79 | 4.9 | This study |