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Table 4 Comparison of selected PHB producing S. cerevisiae strains harbouring the C. necator PHB pathway, consuming different carbon sources

From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose

Strain

Genotype

Carbon source

Growth conditions

PHB yield/carbon source

PHB content (mg/g CDW)

Reference

(mg/g)

(Cmmol/Cmol)

SCKK005

PHB pathway

Glucose

Shake flask

~1.5a

2.1a

4.02

(Kocharin et al. 2013)

SCK006

PHB pathway, ADH2, ALD6, acs L641P, ERG10

Glucose

Shake flask

~5a

7a

15.89

(Kocharin et al. 2013)

SCK032

PHB pathway, xpkA, ack, acs L641P

Glucose

Shake flask

~9a

12.5a

27.86

(Kocharin et al. 2013)

SCK034

PHB pathway, gapN, ADH2, ALD6, acs L641P, ERG10

Glucose

Shake flask

~7a

9.8a

27.52

(Kocharin et al. 2013)

SCKK006

PHB pathway, ADH2, ALD6, acs L641P, ERG10

Glucose

Aerobic, chemostat bioreactor, dilution rate 0.10 h−1

2.79

3.67

5.59

(Kocharin and Nielsen 2013)

Strain RKS

GAL1-10-induced PHB pathway

Galactose/Glucose

Aerobic, batch bioreactor

13.5b

18.8b

75

(Carlson and Srienc 2006)

TMB4443

PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ

Xylose

Shake flask

1.17

1.63

10.4

This study

TMB4443

PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ

Xylose

Aerobic, batch bioreactor

1.99

2.79

4.9

This study

  1. a)Recalculated from graph presented in the respective references, based on total consumption of carbon sources.
  2. b)Calculated based on total consumption of carbon sources.