Skip to main content

Table 4 Comparison of selected PHB producing S. cerevisiae strains harbouring the C. necator PHB pathway, consuming different carbon sources

From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose

Strain Genotype Carbon source Growth conditions PHB yield/carbon source PHB content (mg/g CDW) Reference
(mg/g) (Cmmol/Cmol)
SCKK005 PHB pathway Glucose Shake flask ~1.5a 2.1a 4.02 (Kocharin et al. 2013)
SCK006 PHB pathway, ADH2, ALD6, acs L641P, ERG10 Glucose Shake flask ~5a 7a 15.89 (Kocharin et al. 2013)
SCK032 PHB pathway, xpkA, ack, acs L641P Glucose Shake flask ~9a 12.5a 27.86 (Kocharin et al. 2013)
SCK034 PHB pathway, gapN, ADH2, ALD6, acs L641P, ERG10 Glucose Shake flask ~7a 9.8a 27.52 (Kocharin et al. 2013)
SCKK006 PHB pathway, ADH2, ALD6, acs L641P, ERG10 Glucose Aerobic, chemostat bioreactor, dilution rate 0.10 h−1 2.79 3.67 5.59 (Kocharin and Nielsen 2013)
Strain RKS GAL1-10-induced PHB pathway Galactose/Glucose Aerobic, batch bioreactor 13.5b 18.8b 75 (Carlson and Srienc 2006)
TMB4443 PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ Xylose Shake flask 1.17 1.63 10.4 This study
TMB4443 PHB Pathway, XYL1, XYL2, XKS, PPP↑, gre3Δ Xylose Aerobic, batch bioreactor 1.99 2.79 4.9 This study
  1. a)Recalculated from graph presented in the respective references, based on total consumption of carbon sources.
  2. b)Calculated based on total consumption of carbon sources.