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Table 3 Physiological results of the strains grown aerobically on xylose on shake flasks or bioreactors

From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose

Strain

Growth condition

Growth rate μ max (h −1 )

Yields

PHB titer (mg/L)

Final PHB content (% CDW)

Xylose/sugars consumed (%)

Y sx (g/g Xyl)

Y sEtOH (g/g Xyl)

Y sAc (g/g Xyl)

Y sXylitol (g/g Xyl)

Y sGly (g/g Xyl)

Y sPHB (mg/g Xyl)

TMB 4443

Shake flask

0.231 ± 0.01

0.11 ± 0.02

0.10 ± 0.05

0.14 ± 0.0

0.05 ± 0.03

0.01 ± 0.0

1.17 ± 0.18

45.0 ± 3.54

1.04 ± 0.03

76.8 ± 4.6

TMB 4444

Shake flask

0.249 ± 0.01

0.14 ± 0.0

0.06 ± 0.0

0.14 ± 0.0

0.10 ± 0.0

0.02 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

80.1 ± 3.0

TMB 4443

Bioreactor

0.184 ± 0.01

0.41 ± 0.02

0.004 ± 0.0

0.0 ± 0.0

0.028 ± 0.0

0.0 ± 0.0

1.99 ± 0.15

101.7 ± 7.1

0.49 ± 0.06

100 ± 0

  1. The values reported are calculated for aerobic cultivations on defined media with xylose (50 g L−1) as carbon source. The specific growth rate is calculated for the exponential phase. Yields and titers are calculated from a single time point in the early stationary phase. Reported values represent the mean ± SD of at least two independent cultivations performed. Ysx: biomass yield on xylose, YsEtOH: yield of ethanol on xylose, YsAc: yield of acetate on xylose, YsXylitol: yield of xylitol on xylose, YsGly: yield of glycerol on xylose, YsPHB: yield of PHB on xylose. PHB titer: The volumetric PHB titer. PHB/CDW: The PHB component as percentage of total cell dry weight.