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Table 3 Physiological results of the strains grown aerobically on xylose on shake flasks or bioreactors

From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose

Strain Growth condition Growth rate μ max (h −1 ) Yields PHB titer (mg/L) Final PHB content (% CDW) Xylose/sugars consumed (%)
Y sx (g/g Xyl) Y sEtOH (g/g Xyl) Y sAc (g/g Xyl) Y sXylitol (g/g Xyl) Y sGly (g/g Xyl) Y sPHB (mg/g Xyl)
TMB 4443 Shake flask 0.231 ± 0.01 0.11 ± 0.02 0.10 ± 0.05 0.14 ± 0.0 0.05 ± 0.03 0.01 ± 0.0 1.17 ± 0.18 45.0 ± 3.54 1.04 ± 0.03 76.8 ± 4.6
TMB 4444 Shake flask 0.249 ± 0.01 0.14 ± 0.0 0.06 ± 0.0 0.14 ± 0.0 0.10 ± 0.0 0.02 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 80.1 ± 3.0
TMB 4443 Bioreactor 0.184 ± 0.01 0.41 ± 0.02 0.004 ± 0.0 0.0 ± 0.0 0.028 ± 0.0 0.0 ± 0.0 1.99 ± 0.15 101.7 ± 7.1 0.49 ± 0.06 100 ± 0
  1. The values reported are calculated for aerobic cultivations on defined media with xylose (50 g L−1) as carbon source. The specific growth rate is calculated for the exponential phase. Yields and titers are calculated from a single time point in the early stationary phase. Reported values represent the mean ± SD of at least two independent cultivations performed. Ysx: biomass yield on xylose, YsEtOH: yield of ethanol on xylose, YsAc: yield of acetate on xylose, YsXylitol: yield of xylitol on xylose, YsGly: yield of glycerol on xylose, YsPHB: yield of PHB on xylose. PHB titer: The volumetric PHB titer. PHB/CDW: The PHB component as percentage of total cell dry weight.