Table 2 Primers used in the present study
From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose
Name | Amplification target | Sequence (5‘ to 3’) |
|---|---|---|
TEF1p_f | S. cerevisiae TEF1 promoter | CTAGAGGATCCCCGGGTATAGCTTCAAAATGTTTCTAC |
TEF1p_r | CACGTCAGTCATTTTGTAATTAAAACTTAGATTAG | |
PhaA_f | Codon optimized PhaA ORF from C. necator | GTTTTAATTACAAAATGACTGACGTGGTGATTG |
PhaA_r | CACGTCAGTCATTTTGTAATTAAAACTTAGATTAGATTG | |
TEF1t_f | S. cerevisiae TEF1 terminator | GTTGAAAGAAAGTAAGGAGATTGATAAGACTTTTC |
TEF1t_r | GATATAGAGCTCAGATAGCGCCGATCAAAG | |
TPI1p_f | S. cerevisiae TPI1 promoter | TCTGAGCTCTATATCTAGGAACCCATCAG |
TPI1p_r | CAATCCTTTGCGTCATTTTTAGTTTATGTATGTGTTTTTTG | |
PhaB1_f | Codon optimized PhaB1 ORF from C. necator | CATACATAAACTAAAAATGACGCAAAGGATTGCGTATG |
PhaB1_r | GATAATATTTTTATATAATTATATTATTAACCCATGTGTAAGCCAC | |
TPI1t_f | S. cerevisiae TPI1 terminator | CACATGGGTTAATAATATAATTATATAAAAATATTATCTTCTTTTC |
TPI1t_r | CTAACTAGTTATATAACAGTTGAAATTTGGATAAG | |
GPM1p_f | S. cerevisiae GPM1 promoter | TATATAACTAGTTAGTCGTGCAATGTATGAC |
GPM1p_r | CTTTACCAGTGGCCATTATTGTAATATGTGTGTTTGTTTG | |
PhaC1_f | Codon optimized PhaC1 ORF from C. necator | CATATTACAATAATGGCCACTGGTAAAGG |
PhaC1_r | CATTCATTCTTCAGACTTATGCCTTTGCTTTCACATAC | |
GPM1t_f | S. cerevisiae GPM1 terminator | GAAAGCAAAGGCATAAGTCTGAAGAATGAATGATTTG |
GPM1t_r | TACCGCATCAGGCGCCATATTCGAACTGCCCATTCAG |