Table 1 Plasmids and strains used in this study
From: Engineering of Saccharomyces cerevisiae for the production of poly-3-d-hydroxybutyrate from xylose
Plasmids | Relevant genotype | Reference |
|---|---|---|
YIplac128 | LEU2 | (Gietz and Akio 1988) |
YIpOB8 | pTDH3-XYL1-tADH1; pPGK1-XYL2-tPGK1; URA3 | (Runquist et al. 2010) |
YIpAGS2 | YIplac128; pTEF1-PhaA-tTEF1; pGPM1-PhaB1-pGPM1; pTPI1-PhaC1-tTPI1; LEU2 | This study |
Yeast strains | Relevant genotype | Reference |
TMB 3043 | CEN.PK2-1C; gre3Δ; his3::pPGK1-XKS1-tPGK1, HIS3; tal1::pPGK1-TAL1-tPGK1; tkl1::pPGK1-TKL1-tPGK1; rki1::pPGK1-RKI1-tPGK1; rpe1::pPGK1-RPE1-tPGK1; ura3, leu2 | (Karhumaa et al. 2005) |
TMB 4440 | TMB 3043; ura3::YIpOB8; leu2 | This study |
TMB 4443 | TMB 4440; leu2::YIpAGS2 | This study |
TMB 4444 | TMB 4440; leu2::YIplac128 | This study |