Recombinant glucagon: a differential biological activity

AMB Express

Table 2 Products used for recombinant glucagon expression and respective costs for 1 litre expression

Product	Price (US$)	Amount used	Final price spent (US$)
Ampicillin	134.33	100 mg	2.68
Benzamidine	207.92	180.225 mg	7.49
Enterokinase 1	781.50	1U/20 μg fusioned protein	1094.11*
Enterokinase 2	781.50	1U/1 mg fusion protein	21.87*
Yeast extract	56.60	5 g	1.14
Reduced glutathione	6135.00	3.07 g	14.21
IPTG	86.03	59.57 mg	5.12
KCl	47.54	3 mg	0.0002
KH₂PO₄	107.54	3.67 mg	0.007
NaCl	47.54	5.12 g	0.97
Na₂HPO₄	125.29	21.29 mg	0.005
PMSF	181.13	261.29 mg	9.46
Tips	16.98	15 units	0.25
Glutathione Sepharose	1864.90	4 mL	74.59
Tryptone	128.31	10 g	5.14
TrisHCl	93.59	6.05 g	2.26
Water, equipment and others			9.53
Total with enterokinase 1			1226.96 (/7 = 175.28)**
Total with enterokinase 2			157.72 (/7 = 20.10)**

Prices obtained from the companies Invitrogen, GE and Sigma. *Value may change depending on the concentration used for digestion. In this work, we used manufacturer’s instructions (1U/20 μg), but literature reports (Ehrhardt et al. 2000; SUN et al. 2011) indicate 1U/mg recombinant protein to digestion. Thereby, this concentration was used to calculate this value in this condition. **Total value divided by 7, the recombinant protein total obtained in the 1 litre expression (7 mg in 1 litre) to indicate the value for 1 mg. Basso (2012)