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Table 2 Products used for recombinant glucagon expression and respective costs for 1 litre expression

From: Recombinant glucagon: a differential biological activity

Product Price (US$) Amount used Final price spent (US$)
Ampicillin 134.33 100 mg 2.68
Benzamidine 207.92 180.225 mg 7.49
Enterokinase 1 781.50 1U/20 μg fusioned protein 1094.11*
Enterokinase 2 781.50 1U/1 mg fusion protein 21.87*
Yeast extract 56.60 5 g 1.14
Reduced glutathione 6135.00 3.07 g 14.21
IPTG 86.03 59.57 mg 5.12
KCl 47.54 3 mg 0.0002
KH2PO4 107.54 3.67 mg 0.007
NaCl 47.54 5.12 g 0.97
Na2HPO4 125.29 21.29 mg 0.005
PMSF 181.13 261.29 mg 9.46
Tips 16.98 15 units 0.25
Glutathione Sepharose 1864.90 4 mL 74.59
Tryptone 128.31 10 g 5.14
TrisHCl 93.59 6.05 g 2.26
Water, equipment and others    9.53
Total with enterokinase 1    1226.96 (**/7 = 175.28)
Total with enterokinase 2    157.72 (**/7 = 20.10)
  1. Prices obtained from the companies Invitrogen, GE and Sigma. *Value may change depending on the concentration used for digestion. In this work, we used manufacturer’s instructions (1U/20 μg), but literature reports (Ehrhardt et al. 2000; SUN et al. 2011) indicate 1U/mg recombinant protein to digestion. Thereby, this concentration was used to calculate this value in this condition. **Total value divided by 7, the recombinant protein total obtained in the 1 litre expression (7 mg in 1 litre) to indicate the value for 1 mg. Basso (2012)