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Table 5 Literature comparison

From: Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

Bacteria species Biomass status Chemical Concentration Initial PHA content Purity Recovery Reference
E. coli(rec) Frozen NaOH 0.1 77% 91% 90% Choi and Lee (1999)
E. coli Frozen SDS 0.5% 77% 98% 87% Choi and Lee (1999)
E. coli Frozen NH4OH 0.1 77% 85% 95% Choi and Lee (1999)
E. coli Frozen H2SO4 0.1 77% 79% 87% Choi and Lee (1999)
C.necator Lyophilized NaOH 0.1 38% 97% 97% (Mohammadi et al. 2012a, b)
C.necator Lyophilized NaOH 0.1 60% 80% 90% Anis et al. (2012)
C.necator Lyophilized NH4OH 0.1 60% 60% 62% Anis et al. (2012)
Comamonas Lyophilized NaOH 0.05 34% 89% 97% (Mohammadi et al. 2012a, b)
R.eutropha Lyophilized NaOH N.D. 70% 78% 45% Yang et al. (2011)
R.eutropha Lyophilized SDS 5% 70% 90% 81% Yang et al. (2011)
R.eutropha Lyophilized SDS 1% 50% 87% N.D. Ramsay et al. (1990)
P.putida Lyophilized NaOH 0.1 20% 40% 95% Jiang et al. (2006)
E. coli Oven dried NaOH + SDS 0.1 + 10% 60% 87% 96% Peng et al. (2013)
R.eutropha Fresh SDS 0.5%-20% 75% 97% 92% Kim et al. (2003)
R.eutropha Fresh H2SO4 1 60% 76% 94% Yu and Chen (2006)
A.vinelandii Fresh NH3 1 84% 94% N.D. Page and Cornish (1993)
C.nector Fresh NaOH 0.1 68% 84% 91% Anis et al. (2013)
E. coli Fresh NaOH 0.2 79% 97% 91% Choi and Lee (1999)
P.acidivorans* Fresh NaOH 0.2 68% 89% 97% This study
P.acidivorans* Fresh NH4OH 0.2 68% 65% 78% This study
P.acidivorans* Lyophilized NH4OH 0.2 68% 87% 96% This study
P.acidivorans* Fresh NaOH + SDS 0.2 + 0.2% 68% 99% 95% This study
  1. *Dominant bacterial species in the mixed culture at the cultivation conditions of this study.