Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

AMB Express

Table 5 Literature comparison

Bacteria species	Biomass status	Chemical	Concentration	Initial PHA content	Purity	Recovery	Reference
E. coli(rec)	Frozen	NaOH	0.1	77%	91%	90%	Choi and Lee (1999)
E. coli	Frozen	SDS	0.5%	77%	98%	87%	Choi and Lee (1999)
E. coli	Frozen	NH₄OH	0.1	77%	85%	95%	Choi and Lee (1999)
E. coli	Frozen	H₂SO₄	0.1	77%	79%	87%	Choi and Lee (1999)
C.necator	Lyophilized	NaOH	0.1	38%	97%	97%	(Mohammadi et al. 2012a, b)
C.necator	Lyophilized	NaOH	0.1	60%	80%	90%	Anis et al. (2012)
C.necator	Lyophilized	NH₄OH	0.1	60%	60%	62%	Anis et al. (2012)
Comamonas	Lyophilized	NaOH	0.05	34%	89%	97%	(Mohammadi et al. 2012a, b)
R.eutropha	Lyophilized	NaOH	N.D.	70%	78%	45%	Yang et al. (2011)
R.eutropha	Lyophilized	SDS	5%	70%	90%	81%	Yang et al. (2011)
R.eutropha	Lyophilized	SDS	1%	50%	87%	N.D.	Ramsay et al. (1990)
P.putida	Lyophilized	NaOH	0.1	20%	40%	95%	Jiang et al. (2006)
E. coli	Oven dried	NaOH + SDS	0.1 + 10%	60%	87%	96%	Peng et al. (2013)
R.eutropha	Fresh	SDS	0.5%-20%	75%	97%	92%	Kim et al. (2003)
R.eutropha	Fresh	H₂SO₄	1	60%	76%	94%	Yu and Chen (2006)
A.vinelandii	Fresh	NH₃	1	84%	94%	N.D.	Page and Cornish (1993)
C.nector	Fresh	NaOH	0.1	68%	84%	91%	Anis et al. (2013)
E. coli	Fresh	NaOH	0.2	79%	97%	91%	Choi and Lee (1999)
P.acidivorans*	Fresh	NaOH	0.2	68%	89%	97%	This study
P.acidivorans*	Fresh	NH₄OH	0.2	68%	65%	78%	This study
P.acidivorans*	Lyophilized	NH₄OH	0.2	68%	87%	96%	This study
P.acidivorans*	Fresh	NaOH + SDS	0.2 + 0.2%	68%	99%	95%	This study

*Dominant bacterial species in the mixed culture at the cultivation conditions of this study.