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Table 5 Literature comparison

From: Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

Bacteria species

Biomass status

Chemical

Concentration

Initial PHA content

Purity

Recovery

Reference

E. coli(rec)

Frozen

NaOH

0.1

77%

91%

90%

Choi and Lee (1999)

E. coli

Frozen

SDS

0.5%

77%

98%

87%

Choi and Lee (1999)

E. coli

Frozen

NH4OH

0.1

77%

85%

95%

Choi and Lee (1999)

E. coli

Frozen

H2SO4

0.1

77%

79%

87%

Choi and Lee (1999)

C.necator

Lyophilized

NaOH

0.1

38%

97%

97%

(Mohammadi et al. 2012a, b)

C.necator

Lyophilized

NaOH

0.1

60%

80%

90%

Anis et al. (2012)

C.necator

Lyophilized

NH4OH

0.1

60%

60%

62%

Anis et al. (2012)

Comamonas

Lyophilized

NaOH

0.05

34%

89%

97%

(Mohammadi et al. 2012a, b)

R.eutropha

Lyophilized

NaOH

N.D.

70%

78%

45%

Yang et al. (2011)

R.eutropha

Lyophilized

SDS

5%

70%

90%

81%

Yang et al. (2011)

R.eutropha

Lyophilized

SDS

1%

50%

87%

N.D.

Ramsay et al. (1990)

P.putida

Lyophilized

NaOH

0.1

20%

40%

95%

Jiang et al. (2006)

E. coli

Oven dried

NaOH + SDS

0.1 + 10%

60%

87%

96%

Peng et al. (2013)

R.eutropha

Fresh

SDS

0.5%-20%

75%

97%

92%

Kim et al. (2003)

R.eutropha

Fresh

H2SO4

1

60%

76%

94%

Yu and Chen (2006)

A.vinelandii

Fresh

NH3

1

84%

94%

N.D.

Page and Cornish (1993)

C.nector

Fresh

NaOH

0.1

68%

84%

91%

Anis et al. (2013)

E. coli

Fresh

NaOH

0.2

79%

97%

91%

Choi and Lee (1999)

P.acidivorans*

Fresh

NaOH

0.2

68%

89%

97%

This study

P.acidivorans*

Fresh

NH4OH

0.2

68%

65%

78%

This study

P.acidivorans*

Lyophilized

NH4OH

0.2

68%

87%

96%

This study

P.acidivorans*

Fresh

NaOH + SDS

0.2 + 0.2%

68%

99%

95%

This study

  1. *Dominant bacterial species in the mixed culture at the cultivation conditions of this study.