Table 2 Kinetic parameters of rAaBGL1 for various natural substrates
Substrate | K m (mM) | k cat (s −1 ) | k cat / K m (s −1 •mM −1 ) |
|---|---|---|---|
gentiobiose | 0.52 ± 0.02 | 457 ± 4 | 873 ± 29 |
laminaribiose | 0.41 ± 0.02 | 444 ± 17 | 1080 ± 20 |
laminaritriose | 0.22 ± 0.01 | 337 ± 3 | 1550 ± 80 |
laminaritetraose | 0.72 ± 0.03 | 304 ± 4 | 423 ± 11 |
laminaripentaose | 1.13 ± 0.01 | 285 ± 5 | 251 ± 2 |
cellobiose | 2.06 ± 0.07 | 354 ± 10 | 172 ± 3 |
cellotriose | 0.45 ± 0.02 | 477 ± 3 | 1060 ± 50 |
cellotetraose | 0.32 ± 0.01 | 433 ± 4 | 1340 ± 40 |
cellopentaose | 0.41 ± 0.02 | 433 ± 9 | 1070 ± 30 |