Figure 2

Effects of pH (A) and temperature (B) on the activity (upper panels) and the stability (lower panels) of purified rAaBGL1. To determine the effect of pH on the activity and the stability (A), enzyme was incubated with 1.5 mM pNP-Glc for 10 min in 100 mM following buffers: glycine-HCl, pH 1.9–2.8 (closed circle); sodium acetate, pH 3.4–5.9 (closed triangle); sodium citrate, pH 3.3–6.3 (open circle); sodium phosphate, pH 6.4–7.3 (closed diamond); Tris–HCl pH 6.8–8.9 (closed square); Glycine-NaOH pH 9.5–11.0 (closed inveted triangle). To determine the effect of temperature on the activity and the stability (B), enzyme was incubated with 1.5 mM pNP-Glc at 30–70̊C in 100 mM sodium acetate buffer (pH 5.0). Data are expressed at the mean ± the standard deviation of three independent experiments.