Figure 2

Statistical analysis for detection of proteins specific for degradation of each natural soft biomass type. The total number of proteins identified and the number of proteins involved in saccharification are shown. A total of 372 proteins were initially quantified. To confirm reproducibility, scatter plots of the quantitative values were created, and principal component analysis was performed (Additional files 2, 3). For detection of specific proteins for natural soft biomass degradation, 77 cellulosomal and non-cellulosomal proteins were selected from the 372 proteins identified. To determine which proteins were biomass-specific, empirical Bayes moderated t-tests were performed and volcano plots were generated (Figure 3A).